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fluorescent protein gfp reporter  (OriGene)


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    Structured Review

    OriGene fluorescent protein gfp reporter
    (A) Representative <t>fluorescent</t> images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of <t>GFP-expressing</t> RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.
    Fluorescent Protein Gfp Reporter, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+reporter/PD-L1+(Cd274)+(NM_021893)+Mouse+Tagged+ORF+Clone/pmc13224917-19-31-37
    Average 94 stars, based on 2 article reviews
    fluorescent protein gfp reporter - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer"

    Article Title: PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer

    Journal: Journal of Nuclear Medicine

    doi: 10.2967/jnumed.125.270922

    (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.
    Figure Legend Snippet: (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

    Techniques Used: Incubation, Expressing, In Vitro, Glo Assay

    Related Articles

    Plasmid Preparation:

    Article Title: Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation.
    Article Snippet: Oriented cell divisions are essential for morphogenesis during embryonic development and for homeostasis of adult organisms1.. Divisions within the plane of epithelia, or planar divisions, shape the architecture of epithelial sheets.. Conversely, vertical divisions along the apicobasal axis have been associated with asymmetric fate specification and stratification2,3.

    Article Title: Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification.
    Article Snippet: To evaluate the role of TGF-β/SMAD signalling, BMP-2 (10 ng/ml; Peprotech), BMP-4 (10 ng/ml; Peprotech), Activin-A (10 ng/ml; R&D System) and TGF-β1 (5 ng/ml; Peprotech) were added in the medium. .. To distrupt TGF-β1-dependent SMAD signalling, c-kitpos CSC were transfected with lentiviral vector carrying a shRNA specific for rat Smad2 with a GFP reporter (Smad2shRNA, Origene, Rockville, MD, USA). .. Lentiviral vectors for β-catenin and Smad2 shRNAs were infected at 100 MOI, and 48 h later, GFP expression was assessed by live imaging (FLoyd Cell Imaging Station, Life Technologies, Waltham, MA, USA) and cells were plated for differentiation or sphere formation as described above.

    Article Title: Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification
    Article Snippet: To evaluate the role of TGF- β /SMAD signalling, BMP-2 (10 ng/ml; Peprotech), BMP-4 (10 ng/ml; Peprotech), Activin-A (10 ng/ml; R&D System) and TGF- β 1 (5 ng/ml; Peprotech) were added in the medium. .. To distrupt TGF- β 1-dependent SMAD signalling, c-kit pos CSC were transfected with lentiviral vector carrying a shRNA specific for rat Smad2 with a GFP reporter (Smad2 shRNA , Origene, Rockville, MD, USA). .. Lentiviral vectors for β -catenin and Smad2 shRNAs were infected at 100 MOI, and 48 h later, GFP expression was assessed by live imaging (FLoyd Cell Imaging Station, Life Technologies, Waltham, MA, USA) and cells were plated for differentiation or sphere formation as described above.

    Expressing:

    Article Title: Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation.
    Article Snippet: Oriented cell divisions are essential for morphogenesis during embryonic development and for homeostasis of adult organisms1.. Divisions within the plane of epithelia, or planar divisions, shape the architecture of epithelial sheets.. Conversely, vertical divisions along the apicobasal axis have been associated with asymmetric fate specification and stratification2,3.

    Article Title: Cell cycle expression of FLJ25439, a cytokinesis-associated protein, is mediated by D-box recognition and APC/C-Cdc20 regulated degradation.
    Article Snippet: The midbody is a transient structure forming out of the central spindle at late telophase.. Both the midbody and central spindle have important functions ensuring completion of cytokinesis and defects in this process may lead to genetic diseases, including cancer.. Thus, understanding the mechanisms that control cytokinesis during mitosis can reveal the key components taking part in some of the processes that promote accurate cell division.

    shRNA:

    Article Title: Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation.
    Article Snippet: Oriented cell divisions are essential for morphogenesis during embryonic development and for homeostasis of adult organisms1.. Divisions within the plane of epithelia, or planar divisions, shape the architecture of epithelial sheets.. Conversely, vertical divisions along the apicobasal axis have been associated with asymmetric fate specification and stratification2,3.

    Article Title: Cell cycle expression of FLJ25439, a cytokinesis-associated protein, is mediated by D-box recognition and APC/C-Cdc20 regulated degradation.
    Article Snippet: The midbody is a transient structure forming out of the central spindle at late telophase.. Both the midbody and central spindle have important functions ensuring completion of cytokinesis and defects in this process may lead to genetic diseases, including cancer.. Thus, understanding the mechanisms that control cytokinesis during mitosis can reveal the key components taking part in some of the processes that promote accurate cell division.

    Article Title: Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification.
    Article Snippet: To evaluate the role of TGF-β/SMAD signalling, BMP-2 (10 ng/ml; Peprotech), BMP-4 (10 ng/ml; Peprotech), Activin-A (10 ng/ml; R&D System) and TGF-β1 (5 ng/ml; Peprotech) were added in the medium. .. To distrupt TGF-β1-dependent SMAD signalling, c-kitpos CSC were transfected with lentiviral vector carrying a shRNA specific for rat Smad2 with a GFP reporter (Smad2shRNA, Origene, Rockville, MD, USA). .. Lentiviral vectors for β-catenin and Smad2 shRNAs were infected at 100 MOI, and 48 h later, GFP expression was assessed by live imaging (FLoyd Cell Imaging Station, Life Technologies, Waltham, MA, USA) and cells were plated for differentiation or sphere formation as described above.

    Article Title: Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification
    Article Snippet: To evaluate the role of TGF- β /SMAD signalling, BMP-2 (10 ng/ml; Peprotech), BMP-4 (10 ng/ml; Peprotech), Activin-A (10 ng/ml; R&D System) and TGF- β 1 (5 ng/ml; Peprotech) were added in the medium. .. To distrupt TGF- β 1-dependent SMAD signalling, c-kit pos CSC were transfected with lentiviral vector carrying a shRNA specific for rat Smad2 with a GFP reporter (Smad2 shRNA , Origene, Rockville, MD, USA). .. Lentiviral vectors for β -catenin and Smad2 shRNAs were infected at 100 MOI, and 48 h later, GFP expression was assessed by live imaging (FLoyd Cell Imaging Station, Life Technologies, Waltham, MA, USA) and cells were plated for differentiation or sphere formation as described above.

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 1012 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 1012 TU/mL). ..

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 10 12 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 10 12 TU/mL). ..

    Knockdown:

    Article Title: Cell cycle expression of FLJ25439, a cytokinesis-associated protein, is mediated by D-box recognition and APC/C-Cdc20 regulated degradation.
    Article Snippet: The midbody is a transient structure forming out of the central spindle at late telophase.. Both the midbody and central spindle have important functions ensuring completion of cytokinesis and defects in this process may lead to genetic diseases, including cancer.. Thus, understanding the mechanisms that control cytokinesis during mitosis can reveal the key components taking part in some of the processes that promote accurate cell division.

    Construct:

    Article Title: Cell cycle expression of FLJ25439, a cytokinesis-associated protein, is mediated by D-box recognition and APC/C-Cdc20 regulated degradation.
    Article Snippet: The midbody is a transient structure forming out of the central spindle at late telophase.. Both the midbody and central spindle have important functions ensuring completion of cytokinesis and defects in this process may lead to genetic diseases, including cancer.. Thus, understanding the mechanisms that control cytokinesis during mitosis can reveal the key components taking part in some of the processes that promote accurate cell division.

    Transfection:

    Article Title: Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification.
    Article Snippet: To evaluate the role of TGF-β/SMAD signalling, BMP-2 (10 ng/ml; Peprotech), BMP-4 (10 ng/ml; Peprotech), Activin-A (10 ng/ml; R&D System) and TGF-β1 (5 ng/ml; Peprotech) were added in the medium. .. To distrupt TGF-β1-dependent SMAD signalling, c-kitpos CSC were transfected with lentiviral vector carrying a shRNA specific for rat Smad2 with a GFP reporter (Smad2shRNA, Origene, Rockville, MD, USA). .. Lentiviral vectors for β-catenin and Smad2 shRNAs were infected at 100 MOI, and 48 h later, GFP expression was assessed by live imaging (FLoyd Cell Imaging Station, Life Technologies, Waltham, MA, USA) and cells were plated for differentiation or sphere formation as described above.

    Article Title: Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification
    Article Snippet: To evaluate the role of TGF- β /SMAD signalling, BMP-2 (10 ng/ml; Peprotech), BMP-4 (10 ng/ml; Peprotech), Activin-A (10 ng/ml; R&D System) and TGF- β 1 (5 ng/ml; Peprotech) were added in the medium. .. To distrupt TGF- β 1-dependent SMAD signalling, c-kit pos CSC were transfected with lentiviral vector carrying a shRNA specific for rat Smad2 with a GFP reporter (Smad2 shRNA , Origene, Rockville, MD, USA). .. Lentiviral vectors for β -catenin and Smad2 shRNAs were infected at 100 MOI, and 48 h later, GFP expression was assessed by live imaging (FLoyd Cell Imaging Station, Life Technologies, Waltham, MA, USA) and cells were plated for differentiation or sphere formation as described above.

    FACS:

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 1012 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 1012 TU/mL). ..

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 10 12 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 10 12 TU/mL). ..

    Transduction:

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 1012 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 1012 TU/mL). ..

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 10 12 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 10 12 TU/mL). ..

    Concentration Assay:

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 1012 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 1012 TU/mL). ..

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 10 12 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 10 12 TU/mL). ..

    Control:

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 1012 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 1012 TU/mL). ..

    Article Title: Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation
    Article Snippet: .. Immediately after cell sorting, tdT + cells were transduced with an agrin shRNA lentivirus containing 4 unique 29mer agrin targeting shRNA and a GFP reporter (purchased from Origene; CAT#: TL512313V, and expanded and packaged at the MGH Vector Core Facility, Massachusetts General Hospital Neuroscience Center, Charlestown, MA to have a final concentration of 10 12 TU/ml) or a control scramble shRNA with a GFP reporter (purchased from Origene; CAT# TR30021V, and expanded and packaged at the MGH Vector Core Facility to have a final concentration of 10 12 TU/mL). ..



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    Image Search Results


    A) Recombination strategy for tamoxifen (tmx; cyan)–mediated replacement of the HRas gene (grey, flanked by loxP sites) with HRasG12V (purple) in PlpCreERt2;FR-HRasG12V (pHRsG/+) mice. B) 2-month-old (2MO) mice treated with tmx are subjected to the complex wheel (CW) test, as well as histological and DTI-MRI analyses, 1-16 weeks later. C) Cell populations are analyzed in seven regions throughout the anterior-posterior (I-IV) and lateral “B” - central “C” axes of the corpus callosum (CC), unless otherwise disclosed. D) Immunostaining of coronal sections showing recombinant cells (GFP+, green arrowheads), OLs (GSTpi+, purple arrowheads), and GFP+GSTpi+ recombinant OLs (orange arrowheads) in the CC of WT and pHRsG/+ mice. E) Bar graph showing that the percentage (normalized to dapi) of recombinant cells in WT and pHRsG/+ mice is not significantly different (unpaired Student’s t test; males P= 0.14 and females P= 0.24). Genotype/sex color code and “n” per group in E, G : male WT (green) and pHRsG/+ (orange); female WT (black) and pHRsG/+ (red). F) Immunostaining picture indicating recombinant cells (green arrowheads), microglia (IBA1+, blue arrowheads), and OPCs (PDGFRa+, orange arrowheads) in the CC of WT and pHRsG/+ mice. G) The percentage of IBA+ cells in WT and pHRsG/+ mice (% of age/gender matched WTs) indicates significantly decreased # of microglia in male pHRsG/+ mice (unpaired Student’s t test; P= 0.047). Insets in D and F show the CC region in high magnification (dotted yellow square). Dapi was used to stain nuclei and normalize cell densities per arbitrary units (AU). Scale bar = 25μm. *P < 0.05.

    Journal: bioRxiv

    Article Title: Targeting Nitric Oxide Synthase 2 Reverses Learning Deficits in an Oligodendrocyte-Focused Model of Costello Syndrome

    doi: 10.64898/2026.06.01.729333

    Figure Lengend Snippet: A) Recombination strategy for tamoxifen (tmx; cyan)–mediated replacement of the HRas gene (grey, flanked by loxP sites) with HRasG12V (purple) in PlpCreERt2;FR-HRasG12V (pHRsG/+) mice. B) 2-month-old (2MO) mice treated with tmx are subjected to the complex wheel (CW) test, as well as histological and DTI-MRI analyses, 1-16 weeks later. C) Cell populations are analyzed in seven regions throughout the anterior-posterior (I-IV) and lateral “B” - central “C” axes of the corpus callosum (CC), unless otherwise disclosed. D) Immunostaining of coronal sections showing recombinant cells (GFP+, green arrowheads), OLs (GSTpi+, purple arrowheads), and GFP+GSTpi+ recombinant OLs (orange arrowheads) in the CC of WT and pHRsG/+ mice. E) Bar graph showing that the percentage (normalized to dapi) of recombinant cells in WT and pHRsG/+ mice is not significantly different (unpaired Student’s t test; males P= 0.14 and females P= 0.24). Genotype/sex color code and “n” per group in E, G : male WT (green) and pHRsG/+ (orange); female WT (black) and pHRsG/+ (red). F) Immunostaining picture indicating recombinant cells (green arrowheads), microglia (IBA1+, blue arrowheads), and OPCs (PDGFRa+, orange arrowheads) in the CC of WT and pHRsG/+ mice. G) The percentage of IBA+ cells in WT and pHRsG/+ mice (% of age/gender matched WTs) indicates significantly decreased # of microglia in male pHRsG/+ mice (unpaired Student’s t test; P= 0.047). Insets in D and F show the CC region in high magnification (dotted yellow square). Dapi was used to stain nuclei and normalize cell densities per arbitrary units (AU). Scale bar = 25μm. *P < 0.05.

    Article Snippet: Floating sections were processed for immunodetection using antibodies for the reporter gene GFP (Nacalai Tesque, Kyoto, Japan), and the cell-type markers GSTpi (MBL Ltd, Tokio, Japan), CC1 (Calbiochem, San Diego, CA, USA), NG2 (Millipore, Burlington, MA, USA), PDGFRa (Rn’D systems, Minneapolis, MN), GFAP (Dako, Santa Clara, CA, USA), IBA1 (Wako, Osaka, Japan), and NeuN (Millipore, St. Louis, MO).

    Techniques: Immunostaining, Recombinant, Staining

    (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

    Journal: Journal of Nuclear Medicine

    Article Title: PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer

    doi: 10.2967/jnumed.125.270922

    Figure Lengend Snippet: (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

    Article Snippet: The MOC2( PD-L1 ) cell line was generated in our laboratory to overexpress PD-L1 by transducing MOC2 cells with a lentiviral vector carrying the murine CD274 gene tagged with a green fluorescent protein (GFP) reporter (MR 203953L2; OriGene Technologies).

    Techniques: Incubation, Expressing, In Vitro, Glo Assay